Result: The in vitro intestinal cytotoxicity exhibited by these wild mushrooms in comparison to the edible mushroom indicated their potential gastrointestinal toxicity. GC-MS analysis of the mushrooms facilitated the identification of their volatile chemical profile. The hematological and serum analysis as well as histological examinations were carried out to evaluate their in vivo toxicity. A 28 day sub-acute toxicity study was carried out by orally administering the mushroom extracts to mice at 250 and 500 mg/kg body weight. Further, the hepatotoxicity was assessed by measuring biologically relevant endpoints such as membrane integrity, mitochondrial stress and oxidative status. Methods: The preliminary cytotoxicity of the selected mushrooms was studied in vitro on the intestinal NCM460 and the Chang's liver cell lines on the basis of cell viability. In this perspective, the present study aims to assess the potential effects of ingesting the selected wild mushrooms from regions of the Western Ghats, India. Mycetism being a health concern, deserves scientific attention.
4Herbal and Indian Medicine Research Laboratory, Sri Ramachandra University, Chennai, Indiaīackground: Poisoning by different kinds of toxic mushrooms is unfortunately becoming an increasingly important medical problem, evident from the growing number of reports worldwide since the 1950s.3Phytochemistry and Natural Products, Armats Biotek Training and Research Institute, Chennai, India.2Mushroom Research Lab, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Thiruvananthapuram, India.1Nutrition, Biochemistry and Toxicology, Defence Food Research Laboratory (DRDO), Mysore, India.
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